TUG Recombinant Rabbit Monoclonal Antibody [JE59-09]
cat.: HA720029
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TUG aa 151-350/553. |
| Positive control: | K562 cell lysate, NIH/3T3 cell lysate, rat testis tissue lysates, human kidney tissue, human pancreas tissue, mouse testis tissue, rat kidney tissue. |
| Subcellular location: | Nucleus, Endomembrane system, Endoplasmic reticulum-Golgi intermediate compartment membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9BZE9 Human | Q8VBT9 Mouse Entrez Gene: 691026 Rat |
| Alternative names: | Alveolar soft part sarcoma chromosomal region candidate gene 1 protein Alveolar soft part sarcoma chromosome region candidate 1 (human) Alveolar soft part sarcoma locus ASPC ASPC1_HUMAN ASPCR 1 ASPCR1 ASPL ASPS ASPSCR 1 Aspscr1 FLJ45380 RCC 17 RCC17 renal cell carcinoma gene on chromosome 17 renal cell carcinoma papillary 17 Renal papillary cell carcinoma protein 17 Tether containing a UBX domain for GLUT4 Tether containing UBX domain for GLUT4 TUG UBX domain containing protein 9 UBX domain protein 9 UBX domain-containing protein 9 UBXD 9 UBXD9 UBXN 9 UBXN9 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TUG antibody (HA720029) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TUG antibody (HA720029) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TUG antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TUG antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of TUG on different lysates with Rabbit anti-TUG antibody (HA720029) at 1/5,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720029, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 60.2 kDa Observed band size: 75 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.