TUG Recombinant Rabbit Monoclonal Antibody [JE59-09]
cat.: HA720029
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE59-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 60 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human TUG aa 151-350/553.
Positive control: K562 cell lysate, NIH/3T3 cell lysate, rat testis tissue lysates, mouse kidney tissue, human kidney tissue, human pancreas tissue, mouse testis tissue.
Subcellular location: Nucleus, Endomembrane system, Endoplasmic reticulum-Golgi intermediate compartment membrane.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: Q9BZE9 Human | Q8VBT9 Mouse
Alternative names: Alveolar soft part sarcoma chromosomal region candidate gene 1 protein Alveolar soft part sarcoma chromosome region candidate 1 (human) Alveolar soft part sarcoma locus ASPC ASPC1_HUMAN ASPCR 1 ASPCR1 ASPL ASPS ASPSCR 1 Aspscr1 FLJ45380 RCC 17 RCC17 renal cell carcinoma gene on chromosome 17 renal cell carcinoma papillary 17 Renal papillary cell carcinoma protein 17 Tether containing a UBX domain for GLUT4 Tether containing UBX domain for GLUT4 TUG UBX domain containing protein 9 UBX domain protein 9 UBX domain-containing protein 9 UBXD 9 UBXD9 UBXN 9 UBXN9
Images
HA720029_1.jpg Fig1: Western blot analysis of TUG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720029, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: NIH/3T3 cell lysate
HA720029_2.jpg Fig2: Western blot analysis of TUG on rat testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720029, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA720029_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TUG antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720029_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TUG antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720029_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TUG antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720029_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TUG antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720029, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.