ARPC2 Recombinant Rabbit Monoclonal Antibody [JE58-79]
cat.: HA720030
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE58-79
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 34 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ARPC2 aa 201-300/300.
Positive control: THP-1 cell lysate, Jurkat cell lysate, mouse lung tissue lysate, mouse kidney tissue lysate, rat heart tissue lysate, rat kidney tissue lysate, rat brain tissue, human tonsil tissue, human thyroid tissue, human breast carcinoma tissue, mouse testis tissue.
Subcellular location: Nucleus, Cytoskeleton, Cell projection, Synaptosome.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:100-1:500
Uniprot #: SwissProt: O15144 Human | Q9CVB6 Mouse | P85970 Rat
Alternative names: Actin related protein 2/3 complex subunit 2 34kDa Actin related protein 2/3 complex subunit 2 Actin-related protein 2/3 complex subunit 2 ARC 34 ARC34 Arp2/3 complex 34 kDa subunit ARP2/3 protein compex subunit 34 ARPC 2 Arpc2 ARPC2_HUMAN p34 Arc p34-ARC p34Arc PNAS 139 PNAS139 PRO 2446 PRO2446
Images
HA720030_1.jpg Fig1: Western blot analysis of ARPC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720030, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse kidney tissue lysate
HA720030_2.jpg Fig2: Western blot analysis of ARPC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720030, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat heart tissue lysate
Lane 2: Rat kidney tissue lysate
HA720030_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720030, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720030_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720030, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720030_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720030, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720030_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720030, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720030_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720030, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.