Fukutin Recombinant Rabbit Monoclonal Antibody [JE58-81]
cat.: HA720031
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE58-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Fukutin aa 312-461/461.
Positive control: Jurkat cell lysate, mouse brain tissue lysate, rat cerebellum tissue lysates, rat kidney tissue, human prostate carcinoma tissue, mouse liver tissue.
Subcellular location: Golgi apparatus membrane, Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: O75072 Human | Q8R507 Mouse
Alternative names: CMD1X FCMD FCMD gene FKTN FKTN_HUMAN Fukutin Fukuyama type congenital muscular dystrophy protein Fukuyama-type congenital muscular dystrophy protein LGMD2M MDDGA4 MDDGB4 MDDGC4 MGC126857 MGC134944 MGC134945 MGC138243 OTTHUMP00000021841 patient fukutin
Images
HA720031_1.jpg Fig1: Western blot analysis of Fukutin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720031, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Mouse brain tissue lysate
HA720031_2.jpg Fig2: Western blot analysis of Fukutin on rat cerebellum tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720031, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA720031_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Fukutin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720031, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720031_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-Fukutin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720031, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720031_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Fukutin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720031, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.