ACAT2 Recombinant Rabbit Monoclonal Antibody [JE58-83]
cat.: HA720032
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ACAT2 aa 198-397/397. |
| Positive control: | HepG2 cell lysate, K562 cell lysate, mouse lung tissue lysate, rat testis tissue lysate, rat liver tissue lysate, human prostate tissue, HepG2. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9BWD1 Human | Q8CAY6 Mouse | Q5XI22 Rat |
| Alternative names: | Acat2 acetoacetyl CoA thiolase acetoacetyl Coenzyme A thiolase Acetyl CoA acetyltransferase Acetyl CoA transferase like protein acetyl Coenzyme A acetyltransferase 2 Acetyl-CoA acetyltransferase Acetyl-CoA transferase-like protein Acyl Coenzyme A cholesterol acyltransferase 2 Cytosolic acetoacetyl-CoA thiolase cytosolic SOAT2 THIC_HUMAN |
Images
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Fig1:
Western blot analysis of ACAT2 on different lysates with Rabbit anti-ACAT2 antibody (HA720032) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACAT2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720032) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ACAT2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720032, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysate Lane 3: Mouse lung tissue lysate |
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Fig3:
Western blot analysis of ACAT2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720032, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat testis tissue lysate Lane 2: Rat liver tissue lysate |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ACAT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720032, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of ACAT2 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720032, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.