PDCD7 Recombinant Rabbit Monoclonal Antibody [JE59-06]
cat.: HA720033
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE59-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PDCD7 aa 1-100/485.
Positive control: Jurkat cell lysate, Hela cell lysate, rat stomach tissue lysates, rat kidney tissue, human kidney tissue, human stomach tissue, mouse testis tissue, THP-1.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:500-1:1,000
Uniprot #: SwissProt: Q8N8D1 Human | Q9WTY1 Mouse
Alternative names: Apoptosis-related protein ES18 ES18 HES18 MGC22015 Pdcd7 PDCD7_HUMAN Programmed cell death 7 Programmed cell death protein 7
Images
HA720033_1.jpg Fig1: Western blot analysis of PDCD7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
HA720033_2.jpg Fig2: Western blot analysis of PDCD7 on rat stomach tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA720033_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_7.jpg Fig7: Flow cytometric analysis of PDCD7 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720033, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.