PDCD7 Recombinant Rabbit Monoclonal Antibody [JE59-06]
cat.: HA720033
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE59-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PDCD7 aa 1-100/485.
Positive control: HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse stomach tissue lysate, rat kidney tissue, human kidney tissue, human stomach tissue, mouse testis tissue, THP-1.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:50-1:200
Uniprot #: SwissProt: Q8N8D1 Human | Q9WTY1 Mouse
Entrez Gene: 363082 Rat
Alternative names: Apoptosis-related protein ES18 ES18 HES18 MGC22015 Pdcd7 PDCD7_HUMAN Programmed cell death 7 Programmed cell death protein 7
Images
HA720033_1.jpg Fig1: Western blot analysis of PDCD7 on different lysates with Rabbit anti-PDCD7 antibody (HA720033) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse stomach tissue lysate (30 µg/Lane)

Predicted band size: 55 kDa
Observed band size: 55 kDa

Exposure time: 51 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720033) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720033_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720033_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PDCD7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720033, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.