PDCD7 Recombinant Rabbit Monoclonal Antibody [JE59-06]
cat.: HA720033
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PDCD7 aa 1-100/485. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse stomach tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q8N8D1 Human | Q9WTY1 Mouse Entrez Gene: 363082 Rat |
| Alternative names: | Apoptosis-related protein ES18 ES18 HES18 MGC22015 Pdcd7 PDCD7_HUMAN Programmed cell death 7 Programmed cell death protein 7 |
Images
|
Fig1:
Western blot analysis of PDCD7 on different lysates with Rabbit anti-PDCD7 antibody (HA720033) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse stomach tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720033) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.