USP39 Recombinant Rabbit Monoclonal Antibody [JE59-07]
cat.: HA720034
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human USP39 aa 151-350/565. |
| Positive control: | Hela and Jurkat cell lysates, rat brain tissue, human colon carcinoma tissue, human breast carcinoma tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q53GS9 Human | Q3TIX9 Mouse | B2GV41 Rat |
| Alternative names: | 65K CGI 21 CGI-21 FLJ33136 HSPC332 Inactive ubiquitin specific peptidase 39 Inactive ubiquitin-specific peptidase 39 MGC75069 PRO2855 S. CEREVISIAE, HOMOLOG OF SAD1 SAD1 SAD1 homolog Small nuclear ribonucleoprotein 65kDa (U4/U6.U5) snRNP ASSEMBLY DEFECTIVE 1 SnRNP assembly defective 1 homolog SNRNP65 SNUT2_HUMAN U4/U6.U5 tri snRNP associated 65 kDa protein U4/U6.U5 tri snRNP associated protein 2 U4/U6.U5 tri-snRNP-associated 65 kDa protein U4/U6.U5 tri-snRNP-associated protein 2 Ubiquitin specific peptidase 39 Ubiquitin specific protease 39 USP39 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720034, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720034, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720034, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720034, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of USP39 on different lysates with Rabbit anti-USP39 antibody (HA720034) at 1/2,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720034, 1/2,000 in primary antibody dilution buffer (K1803), 2 hour at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 65 kDa Observed band size: 65 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.