TUB Recombinant Rabbit Monoclonal Antibody [JE58-75]
cat.: HA720035
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TUB aa 1-50/506. |
| Positive control: | SH-SY5Y cell lysates, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Secreted, Cell membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P50607 Human | P50586 Mouse |
| Alternative names: | F10B5.4 rd5 TUB 1 TUB TUB_HUMAN Tubby homologue Tubby protein homolog 1 Tubby protein homolog |
Images
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Fig1: Western blot analysis of TUB on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720035, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TUB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720035, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of TUB was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720035, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.