AK3L1 Recombinant Rabbit Monoclonal Antibody [JE59-30]
cat.: HA720036
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AK3L1 aa 1-100/223. |
| Positive control: | HepG2 cell lysate, A431 cell lysate, A431, human liver tissue. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: P27144 Human |
| Alternative names: | Adenylate kinase 3 like 1 Adenylate kinase 3-like adenylate kinase 4 Adenylate kinase isoenzyme 4, mitochondrial AK3 AK3L1 AK3L2 AK4 ATP AMP transphosphorylase ATP-AMP transphosphorylase GTP:AMP phosphotransferase KAD4_HUMAN MGC166959 mitochondrial adenylate kinase 3 nucleoside-triphosphate adenylate kinase |
Images
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Fig1:
Western blot analysis of AK3L1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720036, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate |
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Fig2: ICC staining of AK3L1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720036, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AK3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720036, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.