CD84 Recombinant Rabbit Monoclonal Antibody [JE59-27]
cat.: HA720037
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD84 aa aa 296-345/345. |
| Positive control: | THP-1 and Daudi cell lysates, human spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UIB8 Human |
| Alternative names: | CD_antigen=CD84 CD84 CD84 antigen CD84 molecule CDw84 Cell surface antigen MAX.3 hCD84 Hly9 beta Hly9-beta Leucocyte differentiation antigen CD84 Leukocyte Antigen Leukocyte antigen CD84 Leukocyte differentiation antigen Leukocyte differentiation antigen CD84 LY9B mCD84 Signaling lymphocytic activation molecule 5 SLAF5_HUMAN SLAM family member 5 SLAMF5 |
Images
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Fig1: Western blot analysis of CD84 on THP-1 and Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720037, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD84 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720037, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.