HLA DMB Recombinant Rabbit Monoclonal Antibody [JE58-74]
cat.: HA720038
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HLA DMB aa 1-100/263. |
| Positive control: | Raji cell lysate, Daudi cell lysate, PANC-1, Daudi. |
| Subcellular location: | Late endosome membrane, Lysosome membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:5,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P28068 Human |
| Alternative names: | class II antigen HLA-DM beta chain class II histocompatibility antigen Class II histocompatibility antigen M beta chain D6S221E DMB HLA class II histocompatibility antigen DM beta chain HLA-DM histocompatibility type, beta chain M beta chain Major histocompatibility complex class II DM beta MHC class II antigen DMB MHC class II antigen HLA DM beta chain MHC class II HLA DMB OTTHUMP00000029257 Really interesting new gene 7 protein RING 7 RING7 |
Images
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Fig1:
Western blot analysis of HLA DMB on different lysates with Rabbit anti-HLA DMB antibody (HA720038) at 1/5,000 dilution. Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720038, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28.9 kDa Observed band size: 29 kDa |
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Fig2: ICC staining of HLA DMB in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720038, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of HLA DMB was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720038, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.