Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE58-66 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 40 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human HFE aa 299-348/348. |
Positive control: | Hela cell lysate, A431 cell lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q30201 Human |
Alternative names: | dJ221C16.10.1 Hemochromatosis Hemochromatosis protein Hereditary hemochromatosis protein Hereditary hemochromatosis protein HLA H HFE 1 HFE HFE_HUMAN HFE1 HH High Fe HLA H HLA-H HLAH MGC:150812 MGC10379 MGC103790 MHC class I like protein HFE MVCD7 TFQTL2 |
Fig1:
Western blot analysis of HFE on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720039, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate |