Glucosidase 2 subunit beta Recombinant Rabbit Monoclonal Antibody [JE58-41]
cat.: HA720042
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE58-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Glucosidase 2 subunit beta aa 479-528/528. |
| Positive control: | Hela cell lysate, A431 cell lysate, MCF-7 cell lysate, human placenta tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P14314 Human |
| Alternative names: | 80K-H protein AGE-binding receptor 2 AGE-R2 G19P1 GLU2B_HUMAN Glucosidase 2 subunit beta Glucosidase II beta subunit Glucosidase II subunit beta Hepatocystin PCLD PKCSH PLD1 PRKCSH Protein kinase C substrate 60.1 kDa protein heavy chain Protein kinase C substrate 80 Kda protein Protein kinase C substrate 80K-H Protein kinase C substrate, 80 Kda protein |
Images
|
Fig1:
Western blot analysis of Glucosidase 2 subunit beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720042, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 3: MCF-7 cell lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Glucosidase 2 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720042, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: ICC staining of Glucosidase 2 subunit beta in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720042, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.