Glucosidase 2 subunit beta Recombinant Rabbit Monoclonal Antibody [JE58-41]
cat.: HA720042
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE58-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Glucosidase 2 subunit beta aa 479-528/528. |
| Positive control: | MCF7 cell lysate, HeLa cell lysate, human placenta tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P14314 Human |
| Alternative names: | 80K-H protein AGE-binding receptor 2 AGE-R2 G19P1 GLU2B_HUMAN Glucosidase 2 subunit beta Glucosidase II beta subunit Glucosidase II subunit beta Hepatocystin PCLD PKCSH PLD1 PRKCSH Protein kinase C substrate 60.1 kDa protein heavy chain Protein kinase C substrate 80 Kda protein Protein kinase C substrate 80K-H Protein kinase C substrate, 80 Kda protein |
Images
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Fig1:
Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (HA720042) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 80 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720042) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (HA720042) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 80 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720042) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Glucosidase 2 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720042, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.