CD79b Recombinant Rabbit Monoclonal Antibody [JE58-34]
cat.: HA720043
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD79b aa 1-100/229. |
| Positive control: | Daudi cell lysate, Raji cell lysate, human lymph nodes tissue, Daudi. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1ug/mL |
| Uniprot #: | SwissProt: P40259 Human |
| Alternative names: | AGM6 B cell antigen receptor complex associated protein beta chain B cell specific glycoprotein B29 B-cell antigen receptor complex-associated protein beta chain B-cell-specific glycoprotein B29 B29 B29/Ig-beta/CD79b CD 79b CD79b CD79b antigen (immunoglobulin associated beta) CD79b antigen CD79b molecule CD79b molecule immunoglobulin associated beta CD79b protein CD79B_HUMAN Ig beta Ig-beta IGB Igbeta Immunoglobulin associated B29 Immunoglobulin associated B29 protein Immunoglobulin associated beta Immunoglobulin associated protein Immunoglobulin-associated B29 protein MGC108607 |
Images
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Fig1:
Western blot analysis of CD79b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720043, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: Raji cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue using anti-CD79b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720043, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of Daudi cells labeling CD79b. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720043, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.