IL-11RA Recombinant Rabbit Monoclonal Antibody [JE53-88]
cat.: HA720044
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE53-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-11RA aa 373-422/422. |
| Positive control: | Mouse spleen tissue lysate, mouse testis tissue lysate, mouse thymus tissue lysate, Jurkat cell lysates, human kidney tissue, Hela. |
| Subcellular location: | Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q14626 Human | Q64385 Mouse |
| Alternative names: | CRSDA hCG_2011440 I11RA_HUMAN IL-11 receptor subunit alpha IL-11R subunit alpha IL-11R-alpha IL-11RA IL11RA Interleukin 11 receptor alpha Interleukin 11 receptor alpha chain Interleukin-11 receptor subunit alpha MGC2146 |
Images
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Fig1:
Western blot analysis of IL-11RA on different lysates with Rabbit anti-IL-11RA antibody (HA720044) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Mouse thymus tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720044) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of IL-11RA on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720044, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IL-11RA antibody (HA720044) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720044) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling IL-11RA with Rabbit anti-IL-11RA antibody (HA720044) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-11RA antibody (HA720044) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5: Flow cytometric analysis of IL-11RA was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720044, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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