Elp3 Recombinant Rabbit Monoclonal Antibody [JE60-10]
cat.: HA720046
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Elp3 aa 1-547/547. |
| Positive control: | Hela cell lysate, K562 cell lysate, mouse small intestine tissue lysate, rat colon tissue lysate, mouse colon tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9H9T3 Human | Q9CZX0 Mouse Entrez Gene: 100362548 Rat |
| Alternative names: | DKFZp761L098 Elongation protein 3 homolog (S. cerevisiae) Elongation protein 3 homolog ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF elongator acetyltransferase complex subunit 3 Elongator complex protein 3 elp3 ELP3_HUMAN FLJ10422 hELP3 Hypothetical protein DKFZp761L098 Kat9 |
Images
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Fig1:
Western blot analysis of Elp3 on different lysates with Rabbit anti-Elp3 antibody (HA720046) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/lane) Lane 2: K-562 cell lysate (10 µg/lane) Lane 3: mouse small intestine tissue lysate (15 µg/lane) Lane 4: rat colon tissue lysate (15 µg/lane) Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720046, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 62 kDa Observed band size: 62 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Elp3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720046, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.