Elp3 Recombinant Rabbit Monoclonal Antibody [JE60-10]
cat.: HA720046
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Elp3 aa 1-547/547. |
| Positive control: | Hela cell lysate, K562 cell lysate, Jurkat cell lysate, 293 cell lysate, mouse small intestine tissue lysate, rat colon tissue lysate, mouse colon tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9H9T3 Human | Q9CZX0 Mouse Entrez Gene: 100362548 Rat |
| Alternative names: | DKFZp761L098 Elongation protein 3 homolog (S. cerevisiae) Elongation protein 3 homolog ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF elongator acetyltransferase complex subunit 3 Elongator complex protein 3 elp3 ELP3_HUMAN FLJ10422 hELP3 Hypothetical protein DKFZp761L098 Kat9 |
Images
|
Fig1:
Western blot analysis of Elp3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720046, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lane 3: Jurkat cell lysate Lane 4: 293 cell lysate Lane 5: Mouse small intestine tissue lysate Lane 6: Rat colon tissue lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Elp3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720046, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.