GBP1 Recombinant Rabbit Monoclonal Antibody [JE60-78]
cat.: HA720049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GBP1 aa 543-592/592. |
| Positive control: | HeLa cell lysate, HeLa treated with 100ng/mL Human IFN gamma for 48 hours cell lysate, HUVEC cell lysate, human spleen tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Cell membrane, Secreted, Golgi apparatus membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P32455 Human | Q01514 Mouse Entrez Gene: 304266 Rat |
| Alternative names: | GBP 1 GBP-1 GBP1 GBP1_HUMAN GTP binding protein 1 GTP-binding protein 1 Guanine nucleotide binding protein 1 Guanine nucleotide-binding protein 1 Guanylate binding protein 1 Guanylate binding protein 1 interferon inducible 67kDa Guanylate binding protein 1 interferon inducible HuGBP 1 HuGBP-1 HuGBP1 Interferon induced guanylate binding protein 1 Interferon-induced guanylate-binding protein 1 OTTHUMP00000012352 |
Images
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Fig1:
Western blot analysis of GBP1 on different lysates with Rabbit anti-GBP1 antibody (HA720049) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Human IFN gamma for 48 hours cell lysate Lane 3: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720049) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-GBP1 antibody (HA720049) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720049) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-GBP1 antibody (HA720049) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720049) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-GBP1 antibody (HA720049) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720049) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.