GBP1 Recombinant Rabbit Monoclonal Antibody [JE60-78]
cat.: HA720049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GBP1 aa 543-592/592. |
| Positive control: | Human small intestine tissue lysates, human small intestine tissue. |
| Subcellular location: | Cell membrane, Secreted, Golgi apparatus membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50 |
| Uniprot #: | SwissProt: P32455 Human |
| Alternative names: | GBP 1 GBP-1 GBP1 GBP1_HUMAN GTP binding protein 1 GTP-binding protein 1 Guanine nucleotide binding protein 1 Guanine nucleotide-binding protein 1 Guanylate binding protein 1 Guanylate binding protein 1 interferon inducible 67kDa Guanylate binding protein 1 interferon inducible HuGBP 1 HuGBP-1 HuGBP1 Interferon induced guanylate binding protein 1 Interferon-induced guanylate-binding protein 1 OTTHUMP00000012352 |
Images
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Fig1: Western blot analysis of GBP1 on human small intestine tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720049, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-GBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720049, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.