Collagen III Recombinant Rabbit Monoclonal Antibody [JE60-79]
cat.: HA720050
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE60-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 139 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Collagen III 101-400/1,466. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, A431 cell lysate, Rat heart tissue lysates, SiHa, mouse heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, rat skin tissue lysate, Hela. |
| Subcellular location: | Extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P02461 Human | P08121 Mouse | P13941 Rat |
| Alternative names: | Alpha 1 type III collagen Alpha1 (III) collagen CO3A1_HUMAN COL 3A1 COL3A1 Collagen alpha 1(III) chain Collagen alpha-1(III) chain Collagen III alpha 1 chain precursor Collagen III alpha 1 polypeptide Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) Collagen type III alpha 1 Collagen type III alpha 1 chain Collagen type III alpha Collagen, fetal EDS4A Ehlers Danlos syndrome type IV, autosomal dominant Fetal collagen Type III collagen |
Images
|
Fig1:
Western blot analysis of Collagen III on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720050, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: A431 cell lysate |
|
Fig2:
Western blot analysis of Collagen III on Rat heart tissue lysates with Rabbit anti-Collagen III antibody (HA720050) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720050) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3: ICC staining of Collagen III in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720050, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4:
Western blot analysis of Collagen III on different lysates with Rabbit anti-Collagen III antibody (HA720050) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Rat skeletal muscle tissue lysate Lane 4: Rat skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig5: Flow cytometric analysis of Collagen III was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720050, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.