USP39 Recombinant Rabbit Monoclonal Antibody [JE60-87]
cat.: HA720051
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JE60-87
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 65 kDa
Isotype: IgG
Immunogen: Recombinant protein within human USP39 aa 466-565/565.
Positive control: Hela cell lysate, 293T cell lysate, K562 cell lysate, rat testis tissue, human colon tissue, human colon carcinoma tissue, human prostate tissue, human prostate carcinoma tissue, human uterus tissue, mouse kidney tissue, 293T, A549, Hela.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q53GS9 Human | Q3TIX9 Mouse | B2GV41 Rat
Alternative names: 65K CGI 21 CGI-21 FLJ33136 HSPC332 Inactive ubiquitin specific peptidase 39 Inactive ubiquitin-specific peptidase 39 MGC75069 PRO2855 S. CEREVISIAE, HOMOLOG OF SAD1 SAD1 SAD1 homolog Small nuclear ribonucleoprotein 65kDa (U4/U6.U5) snRNP ASSEMBLY DEFECTIVE 1 SnRNP assembly defective 1 homolog SNRNP65 SNUT2_HUMAN U4/U6.U5 tri snRNP associated 65 kDa protein U4/U6.U5 tri snRNP associated protein 2 U4/U6.U5 tri-snRNP-associated 65 kDa protein U4/U6.U5 tri-snRNP-associated protein 2 Ubiquitin specific peptidase 39 Ubiquitin specific protease 39 USP39
Images
HA720051_1.jpg Fig1: Western blot analysis of USP39 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720051, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
HA720051_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-USP39 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720051, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720051_9.jpg Fig9: ICC staining of USP39 in 293T cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720051, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA720051_10.jpg Fig10: ICC staining of USP39 in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720051, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA720051_11.jpg Fig11: ICC staining of USP39 in Hela cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720051, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.