RASA1 Recombinant Rabbit Monoclonal Antibody [JE60-89]
cat.: HA720052
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE60-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 116 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RASA1 250-300/1,047.
Positive control: HEK-293 cell lysate, A431 cell lysate, A549 cell lysate, HL-60 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Y79 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse placenta tissue lysate, Rat testis tissue lysate, Rat placenta tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
1:1,000-1:2,000
1:200
Uniprot #: SwissProt: P20936 Human | P50904 Rat
Entrez Gene: 218397 Mouse
Alternative names: CM AVM CMAVM DKFZp434N071 GAP GTPase activating protein GTPase-activating protein OTTHUMP00000222390 OTTHUMP00000222391 OTTHUMP00000222392 OTTHUMP00000222393 p120GAP p120RASGAP PKWS Ras GTPase-activating protein 1 RAS p21 protein activator (GTPase activating protein) 1 Ras p21 protein activator RASA RASA1 RASA1_HUMAN RasGAP Triphosphatase activating protein
Images
HA720052_1.jpg Fig1: Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: U-87 MG cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: Y79 cell lysate (20 µg/Lane)

Predicted band size: 116 kDa
Observed band size: 116 kDa

Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720052_2.jpg Fig2: Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.

Lane 1: Neuro-2a cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Mouse placenta tissue lysate
Lane 5: Rat testis tissue lysate
Lane 6: Rat placenta tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 116 kDa
Observed band size: 116 kDa

Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720052_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720052_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720052_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720052_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (low expression) with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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