Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell |
Clonality: | Monoclonal |
Clone number: | JE60-89 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 116 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human RASA1 250-300/1,047. |
Positive control: | A431 cell lysate, 293 cell lysate, mouse brain tissue lysate, SH-SY5Y. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:100 |
Uniprot #: | SwissProt: P20936 Human | E9PYG6 Mouse |
Alternative names: | CM AVM CMAVM DKFZp434N071 GAP GTPase activating protein GTPase-activating protein OTTHUMP00000222390 OTTHUMP00000222391 OTTHUMP00000222392 OTTHUMP00000222393 p120GAP p120RASGAP PKWS Ras GTPase-activating protein 1 RAS p21 protein activator (GTPase activating protein) 1 Ras p21 protein activator RASA RASA1 RASA1_HUMAN RasGAP Triphosphatase activating protein |
Fig1:
Western blot analysis of RASA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: 293 cell lysate Lane 3: Mouse brain tissue lysate |
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Fig2: ICC staining of RASA1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720052, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |