RASA1 Recombinant Rabbit Monoclonal Antibody [JE60-89]
cat.: HA720052
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE60-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 116 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RASA1 250-300/1,047.
Positive control: A431 cell lysate, 293 cell lysate, mouse brain tissue lysate, SH-SY5Y.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: P20936 Human | E9PYG6 Mouse
Alternative names: CM AVM CMAVM DKFZp434N071 GAP GTPase activating protein GTPase-activating protein OTTHUMP00000222390 OTTHUMP00000222391 OTTHUMP00000222392 OTTHUMP00000222393 p120GAP p120RASGAP PKWS Ras GTPase-activating protein 1 RAS p21 protein activator (GTPase activating protein) 1 Ras p21 protein activator RASA RASA1 RASA1_HUMAN RasGAP Triphosphatase activating protein
Images
HA720052_1.jpg Fig1: Western blot analysis of RASA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse brain tissue lysate
HA720052_2.jpg Fig2: ICC staining of RASA1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720052, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.