ORP1 Recombinant Rabbit Monoclonal Antibody [JE60-99]
cat.: HA720053
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 108/50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ORP1 aa 900-950/950. |
| Positive control: | Mouse heart tissue lysate, rat brain tissue lysate, A549 cell lysate, PC-3 cell lysate, human fallopian tube tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Late endosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9BXW6 Human | Q91XL9 Mouse | Q8K4M9 Rat |
| Alternative names: | FLJ10217 ORP 1 ORP-1 ORP1 OSBL1_HUMAN OSBP 8 OSBP L1 OSBP related protein 1 OSBP-related protein 1 OSBP8 OSBPL 1 OSBPL 1A OSBPL 1B OSBPL1 OSBPL1A OSBPL1B Oxysterol binding protein like 1A Oxysterol binding protein like 1B Oxysterol binding protein related protein 1 Oxysterol-binding protein-related protein 1 |
Images
|
Fig1:
Western blot analysis of ORP1 on different lysates with Rabbit anti-ORP1 antibody (HA720053) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ORP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 108 kDa Observed band size: 108,50 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720053) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ORP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720053, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart tissue lysate Lane 2: Rat brain tissue lysate Lane 3: A549 cell lysate Lane 4: PC-3 cell lysate |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ORP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720053, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.