ORP1 Recombinant Rabbit Monoclonal Antibody [JE60-99]
cat.: HA720053
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ORP1 aa 900-950/950. |
| Positive control: | Mouse heart tissue lysate, Rat brain tissue lysate, A549 cell lysate, PC-3 cell lysate, human testis tissue, mouse testis tissue. |
| Subcellular location: | Late endosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9BXW6 Human | Q91XL9 Mouse | Q8K4M9 Rat |
| Alternative names: | FLJ10217 ORP 1 ORP-1 ORP1 OSBL1_HUMAN OSBP 8 OSBP L1 OSBP related protein 1 OSBP-related protein 1 OSBP8 OSBPL 1 OSBPL 1A OSBPL 1B OSBPL1 OSBPL1A OSBPL1B Oxysterol binding protein like 1A Oxysterol binding protein like 1B Oxysterol binding protein related protein 1 Oxysterol-binding protein-related protein 1 |
Images
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Fig1:
Western blot analysis of ORP1 on different lysates with Rabbit anti-ORP1 antibody (HA720053) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ORP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 108 kDa Observed band size: 108,50 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720053) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ORP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720053, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart tissue lysate Lane 2: Rat brain tissue lysate Lane 3: A549 cell lysate Lane 4: PC-3 cell lysate |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ORP1 antibody (HA720053) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ORP1 antibody (HA720053) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.