Elp4 Recombinant Rabbit Monoclonal Antibody [JE61-03]
cat.: HA720054
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: JE61-03
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Elp4 aa 313-364/424.
Positive control: HeLa cell lysate, NIH/3T3 cell lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q96EB1 Human | Q9ER73 Mouse
Alternative names: C11orf19 dJ68P15A.1 elongation protein 4 homolog (S. cerevisiae) Elongation protein 4 homolog Elongator complex protein 4 ELP4 ELP4_HUMAN FLJ20498 hELP4 PAX6 neighbor gene protein PAX6NEB PAXNEB
Images
HA720054_1.jpg Fig1: Western blot analysis of Elp4 on different lysates with Rabbit anti-Elp4 antibody (HA720054) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: NIH/3T3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.