PPP6C Recombinant Rabbit Monoclonal Antibody [JE61-21]
cat.: HA720056
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE61-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PPP6C aa 255-305/305. |
| Positive control: | Hela cell lysate, mouse spleen tissue lysate, human kidney tissue, Hela, SiHa. |
| Subcellular location: | Mitochondrion, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50 1:50-1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: O00743 Human | Q9CQR6 Mouse |
| Alternative names: | FLJ92648 MGC12249 PP 6 PP6 PP6C PPP 6 PPP 6C PPP6 PPP6_HUMAN Ppp6c Protein phosphatase 6 catalytic subunit Serine/threonine protein phosphatase 6 Serine/threonine protein phosphatase catalytic subunit Serine/threonine-protein phosphatase 6 catalytic subunit |
Images
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Fig1:
Western blot analysis of PPP6C on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720056, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Mouse spleen tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PPP6C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720056, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of PPP6C was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720056, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunocytochemistry analysis of SiHa cells labeling PPP6C with Rabbit anti-PPP6C antibody (HA720056) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP6C antibody (HA720056) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.