SAMM50 Recombinant Rabbit Monoclonal Antibody [JE61-49]
cat.: HA720059
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE61-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SAMM50 aa 341-390/469. |
| Positive control: | A431 cell lysate, HeLa cell lysate, C2C12 cell lysate, mouse pancreas tissue lysate, rat pancreas tissue lysate, human kidney tissue, human liver carcinoma tissue, rat testis tissue. |
| Subcellular location: | Mitochondrion outer membrane, Mitochondrion, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y512 Human | Q8BGH2 Mouse | Q6AXV4 Rat |
| Alternative names: | CGI 51 CGI-51 FLJ35825 FLJ42905 FLJ99036 OMP85 SAM50 SAM50_HUMAN Samm50 sorting and assembly machinery 50kDa sorting and assembly machinery component 50 homolog (S. cerevisiae) Sorting and assembly machinery component 50 homolog sorting and assembly machinery component 50, S. cerevisiae, homolog of TOB55 Transformation-related gene 3 protein TRG-3 YNL026W |
Images
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Fig1:
Western blot analysis of SAMM50 on different lysates with Rabbit anti-SAMM50 antibody (HA720059) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: Mouse pancreas tissue lysate (40 µg/Lane) Lane 5: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720059) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SAMM50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SAMM50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SAMM50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.