WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]
cat.: HA720062
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE61-64 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human WDR68 aa 199-248/342. |
| Positive control: | Hela cell lysate, 293 cell lysate, human prostate tissue, mouse brain tissue, rat epididymis tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P61962 Human | P61963 Mouse Entrez Gene: 303602 Rat |
| Alternative names: | AN11 Dcaf7 DCAF7_HUMAN DDB1- and CUL4-associated factor 7 HAN11 Human anthocyanin Petunia Seven WD repeat protein of the AN11 family 1 SWAN 1 WD repeat domain 68 WD repeat protein An11 homolog WD repeat-containing protein 68 WD repeat-containing protein An11 homolog WDR68 |
Images
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Fig1:
Western blot analysis of WDR68 on different lysates with Rabbit anti-WDR68 antibody (HA720062) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720062, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39 kDa Observed band size: 39 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720062, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720062, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.