Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE61-81 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 84 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human PPM1E aa 514-563/755. |
Positive control: | Daudi cell lysates. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q8WY54 Human |
Alternative names: | Ca(2+)/calmodulin-dependent protein kinase phosphatase N CaMKN CaMKP-N CaMKP-nucleus KIAA1072 Nuclear calmodulin dependent protein kinase phosphatase Partner of PIX 1 Partner of PIX-alpha Partner of PIXA POPX1 PP2CH Ppm1e PPM1E_HUMAN Protein phosphatase 1E Protein phosphatase Mg2+/Mn2+ dependent 1E |
Fig1: Western blot analysis of PPM1E on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720063, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |