PPM1E Recombinant Rabbit Monoclonal Antibody [JE61-81]
cat.: HA720063
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE61-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PPM1E aa 514-563/755. |
| Positive control: | 293T cell lysates;SH-SY5Y cell lysates |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q8WY54 Human |
| Alternative names: | Ca(2+)/calmodulin-dependent protein kinase phosphatase N CaMKN CaMKP-N CaMKP-nucleus KIAA1072 Nuclear calmodulin dependent protein kinase phosphatase Partner of PIX 1 Partner of PIX-alpha Partner of PIXA POPX1 PP2CH Ppm1e PPM1E_HUMAN Protein phosphatase 1E Protein phosphatase Mg2+/Mn2+ dependent 1E |
Images
|
Fig1:
Western blot analysis of PPM1E on different lysates with Rabbit anti-PPM1E antibody (HA720063) at 1/5,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720063, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 84 kDa Observed band size: 84 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.