| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE61-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PPM1E aa 514-563/755. |
| Positive control: | Daudi cell lysates. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q8WY54 Human |
| Alternative names: | Ca(2+)/calmodulin-dependent protein kinase phosphatase N CaMKN CaMKP-N CaMKP-nucleus KIAA1072 Nuclear calmodulin dependent protein kinase phosphatase Partner of PIX 1 Partner of PIX-alpha Partner of PIXA POPX1 PP2CH Ppm1e PPM1E_HUMAN Protein phosphatase 1E Protein phosphatase Mg2+/Mn2+ dependent 1E |
|
Fig1: Western blot analysis of PPM1E on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720063, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |