NDRG3 Recombinant Rabbit Monoclonal Antibody [JE61-18]
cat.: HA720068
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE61-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NDRG3 aa 1-35/375. |
| Positive control: | LNCaP cell lysate, Raji cell lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, rat testis tissue lysate, human kidney tissue, human small intestine tissue, PC-3M. |
| Subcellular location: | Cytoplasm, extracellular exosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000-1:5,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UGV2 Human | Q9QYF9 Mouse | Q6AYR2 Rat |
| Alternative names: | FLJ13556 N myc downstream regulated gene 3 N-myc downstream-regulated gene 3 protein NDRG family member 3 NDRG3 NDRG3_HUMAN Protein Ndr3 Protein NDRG3 |
Images
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Fig1:
Western blot analysis of NDRG3 on different lysates with Rabbit anti-NDRG3 antibody (HA720068) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NDRG3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 46 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720068) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of NDRG3 on different lysates with Rabbit anti-NDRG3 antibody (HA720068) at 1/5,000 dilution. Lane 1: LNCaP cell lysate Lane 2: Raji cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 46 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720068) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720068, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720068, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of NDRG3 was done on PC-3M cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720068, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.