NK-p44 Recombinant Rabbit Monoclonal Antibody [JE61-04]
cat.: HA720070
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE61-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NK-p44 aa 1-100/276. |
| Positive control: | K562 cell lysate, HL-60 cell lysate, human small intestine tissue, mouse kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O95944 Human Entrez Gene: 367216 Rat |
| Alternative names: | Activating NK receptor NKp44 CD336 CD336 antigen dJ149M18.1 LY95 Lymphocyte antigen 95 (activating NK receptor; NK p44) Lymphocyte antigen 95 homolog (activating NK receptor; NK p44) Lymphocyte antigen 95 homolog Lymphocyte antigen 95, mouse, homolog of Natural cytotoxicity triggering receptor 2 Natural killer cell p44 related protein Natural killer cell p44-related protein NCR2 NCTR2_HUMAN NK cell activating receptor (NKp44) NK cell activating receptor NK cell-activating receptor NK p44 NK-p44 NKp44 |
Images
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Fig1:
Western blot analysis of NK-p44 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720070, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: HL-60 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NK-p44 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720070, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NK-p44 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720070, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.