Mitofusin 2 Recombinant Rabbit Monoclonal Antibody [JE59-66]
cat.: HA720073
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Mitofusin 2 aa 51-100/757. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293 cell lysate. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: O95140 Human | Q80U63 Mouse | Q8R500 Rat |
| Alternative names: | CMT2A CMT2A2 CPRP 1 CPRP1 EC 3.6.5.- Fzo HSG hyperplasia suppressor gene Hypertension related protein 1 KIAA0214 MARF MFN 2 Mfn2 MFN2_HUMAN Mitochondrial assembly regulatory factor Mitofusin-2 Mitofusin2 Transmembrane GTPase MFN2 |
Images
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Fig1:
Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody (HA720073) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 86 kDa Observed band size: 75 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Mitofusin 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720073, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: 293 cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.