TCPTP Recombinant Rabbit Monoclonal Antibody [JE57-07]
cat.: HA720074
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE57-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TCPTP aa 1-100/415. |
| Positive control: | K562 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HL-60 cell lysate, Jurkat cell lysate, Daudi. |
| Subcellular location: | Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P17706 Human | Q06180 Mouse | P35233 Rat |
| Alternative names: | Protein tyrosine phosphatase non receptor type 2 PTN2 PTN2_HUMAN PTPN 2 Ptpn2 PTPT T cell protein tyrosine phosphatase T-cell protein-tyrosine phosphatase TC PTP TCELLPTP TCPTP Tyrosine protein phosphatase non receptor type 2 Tyrosine-protein phosphatase non-receptor type 2 |
Images
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Fig1:
Western blot analysis of TCPTP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720074, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Lane 4: HL-60 cell lysate Lane 5: Jurkat cell lysate |
|
Fig2:
Flow cytometric analysis of Daudi cells labeling TCPTP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA720074, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.