TCPTP Recombinant Rabbit Monoclonal Antibody [JE57-07]
cat.: HA720074
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE57-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TCPTP aa 1-100/415. |
| Positive control: | K562 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HL-60 cell lysate, Jurkat cell lysate, Daudi. |
| Subcellular location: | Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:5,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P17706 Human | Q06180 Mouse | P35233 Rat |
| Alternative names: | Protein tyrosine phosphatase non receptor type 2 PTN2 PTN2_HUMAN PTPN 2 Ptpn2 PTPT T cell protein tyrosine phosphatase T-cell protein-tyrosine phosphatase TC PTP TCELLPTP TCPTP Tyrosine protein phosphatase non receptor type 2 Tyrosine-protein phosphatase non-receptor type 2 |
Images
|
Fig1:
Western blot analysis of TCPTP on different lysates with Rabbit anti-TCPTP antibody (HA720074) at 1/5,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720074, 1/5,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48.5 kDa Observed band size: 45, 48 kDa |
|
Fig2:
Flow cytometric analysis of Daudi cells labeling TCPTP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA720074, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.