Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE40-35 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 36 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human FHL1 aa 6-55/323. |
Positive control: | Jurkat cell lysate, rat testis tissue lysate. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: Q13642 Human | Q9WUH4 Rat |
Alternative names: | bA535K18.1 FHL 1 FHL 1B FHL-1 FHL1 FHL1 protein FHL1_HUMAN FHL1A FHL1B FLH1A Four and a half LIM domains 1 Four and a half LIM domains protein 1 Four and a half Lin11 Isl 1 and Mec 3 domains 1 four-and-a-half Lin11 Isl-1 and Mec-3 KYO T KYOT, mouse, homolog of LIM protein SLIMMER MGC111107 RAM14-1 RBMX1A RBMX1B RBP associated molecule 14-1 RP11-535K18.1 Skeletal muscle LIM protein 1 Skeletal muscle LIM-protein 1 SLIM 1 SLIM SLIM-1 SLIM1 SLIMMER XMPMA |
Fig1:
Western blot analysis of FHL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720075, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Rat testis tissue lysate |