FHL1 Recombinant Rabbit Monoclonal Antibody [JE40-35]
cat.: HA720075
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FHL1 aa 6-55/323. |
| Positive control: | Human kidney tissue, mouse kidney tissue, rat kidney tissue, Mouse skeletal muscle tissue lysate, Mouse colon tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: Q13642 Human | P97447 Mouse | Q9WUH4 Rat |
| Alternative names: | bA535K18.1 FHL 1 FHL 1B FHL-1 FHL1 FHL1 protein FHL1_HUMAN FHL1A FHL1B FLH1A Four and a half LIM domains 1 Four and a half LIM domains protein 1 Four and a half Lin11 Isl 1 and Mec 3 domains 1 four-and-a-half Lin11 Isl-1 and Mec-3 KYO T KYOT, mouse, homolog of LIM protein SLIMMER MGC111107 RAM14-1 RBMX1A RBMX1B RBP associated molecule 14-1 RP11-535K18.1 Skeletal muscle LIM protein 1 Skeletal muscle LIM-protein 1 SLIM 1 SLIM SLIM-1 SLIM1 SLIMMER XMPMA |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-FHL1 antibody (HA720075) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720075) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-FHL1 antibody (HA720075) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720075) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-FHL1 antibody (HA720075) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720075) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Western blot analysis of FHL1 on different lysates with Rabbit anti-FHL1 antibody (HA720075) at 1/2,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Mouse colon tissue lysate Lane 3: Rat skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720075, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36 kDa Observed band size: 36 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.