SOS1 Recombinant Rabbit Monoclonal Antibody [JE30-06]
cat.: HA720077
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE30-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 152 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SOS1 aa 1-150/1,333. |
| Positive control: | A549 cell lysate, A431 cell lysate, 293T cell lysate, HeLa cell lysate, K-562 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse colon tissue. |
| Subcellular location: | Cytosol, plasma membrane, cytoplasm, neuronal cell body, postsynaptic density. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:500 |
| Uniprot #: | SwissProt: Q07889 Human | Q62245 Mouse Entrez Gene: 313845 Rat |
| Alternative names: | alternate SOS1 GF1 GGF1 GINGF gingival fibromatosis gingival fibromatosis hereditary 1 Guanine nucleotide exchange factor HGF NS4 Son of sevenless homolog 1 (Drosophila) Son of sevenless homolog 1 SOS Ras/Rac guanine nucleotide exchange factor 1 SOS-1 Sos1 SOS1_HUMAN |
Images
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Fig1:
Western blot analysis of SOS1 on different lysates with Rabbit anti-SOS1 antibody (HA720077) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 152 kDa Observed band size: 152 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720077) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SOS1 antibody (HA720077) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720077) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.