GC1q R Recombinant Rabbit Monoclonal Antibody [JE60-96]
cat.: HA720078
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE60-96
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human GC1q R aa 200-249/282.
Positive control: PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Hela cell lysate, A549 cell lysate, mouse spleen tissue lysate, human colon carcinoma tissue, human tonsil tissue, mouse small intestine tissue, rat testis tissue.
Subcellular location: Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IHC-P
1:1,000-1:5,000
1:50-1:200
Uniprot #: SwissProt: Q07021 Human | O35658 Mouse | O35796 Rat
Alternative names: ASF/SF2 associated protein p32 C1q globular domain binding protein C1qBP C1QBP_HUMAN Complement component 1 q subcomponent binding protein Complement component 1 Q subcomponent binding protein mitochondrial Complement component 1 Q subcomponent-binding protein, mitochondrial GC1Q R GC1q R protein GC1q-R protein GC1QBP GC1QR globular domain of, C1q, receptor for Glycoprotein gC1qBP HABP 1 HABP1 Hyaluronan binding protein 1 Hyaluronan-binding protein 1 Mitochondrial matrix protein p32 p32 p32 splicing factor p33 Pre mrna splicing factor SF2 P32 subunit precursor SF2p32 Splicing factor SF2 associated protein
Images
HA720078_1.jpg Fig1: Western blot analysis of GC1q R on different lysates with Rabbit anti-GC1q R antibody (HA720078) at 1/5,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-GC1q R KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720078) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720078_2.jpg Fig2: Western blot analysis of GC1q R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBSA for 1 hour at room temperature. The primary antibody (HA720078, 1/500) was used in 5% NFTM/TBSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-12 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: Hela cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse spleen tissue lysate
HA720078_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720078_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720078_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720078_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.