IRAK-1 Recombinant Rabbit Monoclonal Antibody [JE60-03]
cat.: HA720079
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE60-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IRAK-1 aa 201-500/712. |
| Positive control: | A549 cell lysate, HeLa cell lysate, human colon cancer tissue. |
| Subcellular location: | Cytoplasm, Lipid droplet, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:100-1:200 1-2μg/sample |
| Uniprot #: | SwissProt: P51617 Human | Q62406 Mouse |
| Alternative names: | AA48924 Il1rak Interleukin 1 receptor associated kinase 1 Interleukin-1 receptor-associated kinase 1 IRAK IRAK-1 Irak1 IRAK1-S IRAK1_HUMAN mPLK OTTHUMP00000026014 OTTHUMP00000026015 OTTHUMP00000026020 OTTHUMP00000180621 Pelle Pelle homolog Pelle-like protein kinase Plpk |
Images
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Fig1:
Western blot analysis of IRAK-1 on different lysates with Rabbit anti-IRAK-1 antibody (HA720079) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si IRAK-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 77 kDa Observed band size: 75/77 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720079) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of IRAK-1 on different lysates with Rabbit anti-IRAK-1 antibody (HA720079) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 77 kDa Observed band size: 75 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720079) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-IRAK-1 antibody (HA720079) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720079) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
IRAK-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA720079 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA720079 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA720079 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA720079 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.