HMGCS2 Recombinant Rabbit Monoclonal Antibody [JE54-76]
cat.: HA720083
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE54-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HMGCS2 aa 459-508/508. |
| Positive control: | Caco-2 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat liver tissue, human kidney tissue, Caco-2. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:50-1:200 1:100 |
| Uniprot #: | SwissProt: P54868 Human | P54869 Mouse | P22791 Rat |
| Alternative names: | 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 3 hydroxy 3 methylglutaryl coenzyme A synthase 3-hydroxy-3-methylglutaryl coenzyme A synthase HMCS2_HUMAN HMG CoA synthase HMG-CoA synthase HMGCS 2 HMGCS2 Hydroxymethylglutaryl CoA synthase Hydroxymethylglutaryl CoA synthase mitochondrial Hydroxymethylglutaryl-CoA synthase mitochondrial |
Images
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Fig1:
Western blot analysis of HMGCS2 on different lysates with Rabbit anti-HMGCS2 antibody (HA720083) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 57 kDa Observed band size: 48 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720083) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-HMGCS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720083, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGCS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720083, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Caco-2 (positive) and HCT 116 (negative) labeling HMGCS2 with Rabbit anti-HMGCS2 antibody (HA720083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HMGCS2 antibody (HA720083) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.