Cystatin-B Recombinant Rabbit Monoclonal Antibody [JE63-34]
cat.: HA720084
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cystatin-B aa 49-98/98. |
| Positive control: | PC-12 cell lysates, rat bladder tissue, rat tongue tissue, human esophagus tissue, PC-3M. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P04080 Human | P01041 Rat |
| Alternative names: | CHROW21 CPI B CPI-B CST 6 CST6 CSTB Cystatin B (stefin B) Cystatin B Cystatin-B CYTB CYTB_HUMAN EPM1 EPM1A Liver thiol proteinase inhibitor PME Stefin-B STF B STFB ULD |
Images
|
Fig1:
Western blot analysis of Cystatin-B on different lysates with Rabbit anti-Cystatin-B antibody (HA720084) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cystatin-B KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 15 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720084) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Cystatin-B on PC-12 cell lysates with Rabbit anti-Cystatin-B antibody (HA720084) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720084) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Cystatin-B antibody (HA720084) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat tongue tissue with Rabbit anti-Cystatin-B antibody (HA720084) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Cystatin-B antibody (HA720084) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Flow cytometric analysis of PC-3M cells labeling Cystatin-B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA720084, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.