AMSH Recombinant Rabbit Monoclonal Antibody [JE63-41]
cat.: HA720085
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AMSH aa 151-200/424. |
| Positive control: | MCF-7 cell lysate, 293T cell lysate, HepG2 cell lysate, Hela cell lysate, SW480 cell lysate. |
| Subcellular location: | Cytoplasm, Endosome, Membrane, Nucleus. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: O95630 Human |
| Alternative names: | AMSH Associated molecule with the SH3 domain of STAM Endosome associated ubiquitin isopeptidase Endosome-associated ubiquitin isopeptidase MGC126516 MGC126518 MICCAP STABP_HUMAN STAM binding protein STAM-binding protein Stambp |
Images
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Fig1:
Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: SW480 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 50kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.