AMSH Recombinant Rabbit Monoclonal Antibody [JE63-41]
cat.: HA720085
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AMSH aa 151-200/424. |
| Positive control: | MCF-7 cell lysate, 293T cell lysate, HepG2 cell lysate, Hela cell lysate, SW480 cell lysate. |
| Subcellular location: | Nucleus, Membrane, Cytoplasm, Early endosome. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: O95630 Human |
| Alternative names: | AMSH Associated molecule with the SH3 domain of STAM Endosome associated ubiquitin isopeptidase Endosome-associated ubiquitin isopeptidase MGC126516 MGC126518 MICCAP STABP_HUMAN STAM binding protein STAM-binding protein Stambp |
Images
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Fig1:
Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720085, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.