NDUFAB1 Recombinant Rabbit Monoclonal Antibody [JE65-76]
cat.: HA720088
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE65-76
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG
Immunogen: Recombinant protein within human NDUFAB1 aa 57-156/156.
Positive control: Human small intestine tissue lysates, human endometrium tissue, HepG2.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:100
1:500-1:1,000
Uniprot #: SwissProt: O14561 Human
Alternative names: ACP ACPM_HUMAN Acyl carrier protein Acyl carrier protein mitochondrial CI SDAP CI-SDAP FASN 2A FASN2A MGC65095 Mitochondrial acyl carrier protein mitochondrial NADH dehydrogenase (ubiquinone) 1 alpha/beta subcomplex 1 8kDa NADH dehydrogenase (ubiquinone) 1 alpha/beta subcomplex 1 NADH ubiquinone oxidoreductase 9.6 kDa subunit NADH ubiquinone oxidoreductase SDAP subunit NADH-ubiquinone oxidoreductase 9.6 kDa subunit NDUFAB 1 ndufab1 SDAP
Images
HA720088_1.jpg Fig1: Western blot analysis of NDUFAB1 on human small intestine tissue lysates with Rabbit anti-NDUFAB1 antibody (HA720088) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 16 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720088) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA720088_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-NDUFAB1 antibody (HA720088) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720088) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720088_3.jpg Fig3: Flow cytometric analysis of HepG2 cells labeling NDUFAB1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA720088, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.