DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
cat.: HA720090
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE60-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 107 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DDX58 aa 250-450/925. |
| Positive control: | HUVEC cell lysates. |
| Subcellular location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junction. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: O95786 Human |
| Alternative names: | Ddx58 DDX58_HUMAN DEAD (Asp Glu Ala Asp) box polypeptide 58 DEAD (Asp Glu Ala Asp/His) box polypeptide DEAD box protein 58 DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 DKFZp434J1111 DKFZp686N19181 FLJ13599 Probable ATP dependent RNA helicase DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid inducible gene 1 protein Retinoic acid-inducible gene 1 protein Retinoic acid-inducible gene I protein RIG I Rig-1 RIG-I RIG1 rigi RLR 1 RNA helicase RNA helicase RIG I SGMRT2 |
Images
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Fig1:
Western blot analysis of DDX58 on HUVEC cell lysates with Rabbit anti-DDX58 antibody (HA720090) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720090) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.