| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE60-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HATH1 aa 1-50/354. |
| Positive control: | HepG2 cell lysate, SW480 cell lysate, SW620 cell lysate, HCT 116 cell lysate, human brain tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q92858 Human | P48985 Mouse | D3ZQL9 Rat |
| Alternative names: | ATH1 ATOH1 ATOH1_HUMAN Atonal homolog 1 (Drosophila) Atonal homolog 1 bHLHa14 Class A basic helix-loop-helix protein 14 hATH1 Helix loop helix protein hATH 1 Helix-loop-helix protein hATH-1 MATH 1 MATH1 Protein atonal homolog 1 |
|
Fig1:
Western blot analysis of MATH1 / HATH1 on different lysates with Rabbit anti-MATH1 / HATH1 antibody (HA720091) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SW480 cell lysate Lane 3: SW620 cell lysate Lane 4: HCT 116 cell lysate Lane 5: Human brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720091) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |