ULBP1 Recombinant Rabbit Monoclonal Antibody [JE65-75]
cat.: HA720092
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ULBP1 aa 1-50/244. |
| Positive control: | SiHa cell lysates, Jurkat cell lysates. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum, Membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9BZM6 Human |
| Alternative names: | Alcan beta ALCAN-beta N2DL-1 N2DL1 N2DL1_HUMAN NKG2D ligand 1 NKG2D ligand 1 precursor NKG2DL1 RAET1I Retinoic acid early transcript 1I UL16 UL16 binding protein 1 UL16-binding protein 1 ULBP1 |
Images
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Fig1:
Western blot analysis of ULBP1 on SiHa cell lysates with Rabbit anti-ULBP1 antibody (HA720092) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720092) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ULBP1 on Jurkat cell lysates with Rabbit anti-ULBP1 antibody (HA720092) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720092) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.