| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE61-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ITPA aa 101-150/194. |
| Positive control: | K-562 cell lysate, HepG2 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9BY32 Human |
| Alternative names: | C20orf37 dJ794I6.3 HLC14-06-P Inosine triphosphatase (nucleoside triphosphate pyrophosphatase) Inosine triphosphatase inosine triphosphatase-A Inosine triphosphate pyrophosphatase Inosine triphosphate pyrophosphohydrolase Itpa ITPA_HUMAN ITPase My049 My049 protein Non canonical purine NTP pyrophosphatase Non standard purine NTP pyrophosphatase NTPase nucleoside triphosphate diphosphatase Nucleoside triphosphate pyrophosphatase OK/SW-cl.9 OTTHUMP00000030094 OTTHUMP00000160459 Putative oncogene protein hlc14-06-p |
|
Fig1:
Western blot analysis of ITPA on different lysates with Rabbit anti-ITPA antibody (HA720093) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720093) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ITPA on different lysates with Rabbit anti-ITPA antibody (HA720093) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ITPA KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720093) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |