ITPA Recombinant Rabbit Monoclonal Antibody [JE61-67]
cat.: HA720093
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE61-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ITPA aa 101-150/194. |
| Positive control: | U937 cell lysate, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9BY32 Human |
| Alternative names: | C20orf37 dJ794I6.3 HLC14-06-P Inosine triphosphatase (nucleoside triphosphate pyrophosphatase) Inosine triphosphatase inosine triphosphatase-A Inosine triphosphate pyrophosphatase Inosine triphosphate pyrophosphohydrolase Itpa ITPA_HUMAN ITPase My049 My049 protein Non canonical purine NTP pyrophosphatase Non standard purine NTP pyrophosphatase NTPase nucleoside triphosphate diphosphatase Nucleoside triphosphate pyrophosphatase OK/SW-cl.9 OTTHUMP00000030094 OTTHUMP00000160459 Putative oncogene protein hlc14-06-p |
Images
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Fig1: Western blot analysis of ITPA on U937 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720093, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ITPA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720093, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.