ITPA Recombinant Rabbit Monoclonal Antibody [JE61-67]
cat.: HA720093
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE61-67
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ITPA aa 101-150/194.
Positive control: K-562 cell lysate, HepG2 cell lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9BY32 Human
Alternative names: C20orf37 dJ794I6.3 HLC14-06-P Inosine triphosphatase (nucleoside triphosphate pyrophosphatase) Inosine triphosphatase inosine triphosphatase-A Inosine triphosphate pyrophosphatase Inosine triphosphate pyrophosphohydrolase Itpa ITPA_HUMAN ITPase My049 My049 protein Non canonical purine NTP pyrophosphatase Non standard purine NTP pyrophosphatase NTPase nucleoside triphosphate diphosphatase Nucleoside triphosphate pyrophosphatase OK/SW-cl.9 OTTHUMP00000030094 OTTHUMP00000160459 Putative oncogene protein hlc14-06-p
Images
HA720093_1.jpg Fig1: Western blot analysis of ITPA on different lysates with Rabbit anti-ITPA antibody (HA720093) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: HepG2 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720093) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720093_2.jpg Fig2: Western blot analysis of ITPA on different lysates with Rabbit anti-ITPA antibody (HA720093) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ITPA KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 120 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720093) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.