alpha 1a Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody [JE62-50]
cat.: HA720096
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE62-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human alpha 1a Adrenergic Receptor aa 200-250/466. |
| Positive control: | Jurkat cell lysate, SH-SY5Y cell lysate, PC-3M cell lysate, mouse lung tissue lysate, SH-SY5Y, SiHa, rat heart tissue. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus membrane, caveola. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:100 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P35348 Human | P97718 Mouse | P43140 Rat |
| Alternative names: | ADA1A_HUMAN ADRA1A Adra1c ADRA1L1 Adrenergic alpha1A receptor Adrenergic alpha1C receptor Adrenergic receptor alpha 1a Adrenergic receptor alpha 1c Adrenergic, alpha 1A , receptor variant 1 Adrenergic, alpha 1A , receptor variant 11 Adrenergic, alpha 1A , receptor variant 3 Adrenergic, alpha 1A , receptor variant 5 Adrenergic, alpha 1A , receptor variant 8 Adrenoceptor alpha 1A Alpha 1A adrenoreceptor Alpha 1C adrenergic receptor Alpha adrenergic receptor 1c Alpha-1A adrenergic receptor Alpha-1A adrenoceptor Alpha-1A adrenoreceptor Alpha-1C adrenergic receptor Alpha-adrenergic receptor 1c Alpha1A adrenergic receptor ALPHA1AAR G protein coupled receptor |
Images
|
Fig1:
Western blot analysis of alpha 1a Adrenergic Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720096, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: PC-3M cell lysate Lane 4: Mouse lung tissue lysate |
|
Fig2: ICC staining of alpha 1a Adrenergic Receptor in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720096, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of alpha 1a Adrenergic Receptor in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720096, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-alpha 1a Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720096, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Flow cytometric analysis of alpha 1a Adrenergic Receptor was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720096, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.