Sterol carrier protein 2 Recombinant Rabbit Monoclonal Antibody [JE62-79]
cat.: HA720099
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Sterol carrier protein 2 aa 14-64/547. |
| Positive control: | SK-Br-3 cell lysate, THP-1 cell lysate, HL-60 cell lysate, human small intestine tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum, Mitochondrion, Peroxisome. |
| Recommended Dilutions:
WB IHC-P |
1:5000 1:100-1:500 |
| Uniprot #: | SwissProt: P22307 Human | P32020 Mouse |
| Alternative names: | DKFZp686C12188 DKFZp686D11188 NLTP NLTP_HUMAN Non-specific lipid-transfer protein Nonspecific lipid transfer protein NSL TP NSL-TP OTTHUMP00000010488 OTTHUMP00000231766 OTTHUMP00000231767 OTTHUMP00000231768 OTTHUMP00000231769 OTTHUMP00000231770 OTTHUMP00000231772 OTTHUMP00000231773 OTTHUMP00000231774 OTTHUMP00000231776 OTTHUMP00000234662 Propanoyl CoA C acyltransferase Propanoyl-CoA C-acyltransferase SCP 2 SCP chi SCP X SCP-2 SCP-chi SCP-X SCP2 SCPchi SCPX Sterol carrier protein 2 Sterol carrier protein X |
Images
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Fig1:
Western blot analysis of Sterol carrier protein 2 on different lysates with Rabbit anti-Sterol carrier protein 2 antibody (HA720099) at 1/5,000 dilution. Lane 1: SK-BR-3 (Human breast cancer cell) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720099, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa Observed band size: 59 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Sterol carrier protein 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720099, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Sterol carrier protein 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720099, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.