NCAPD2 Recombinant Rabbit Monoclonal Antibody [JE62-22]
cat.: HA720100
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 157 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NCAPD2 aa 51-200/1,401. |
| Positive control: | Hela cell lysate, HL-60 cell lysate, SiHa cell lysate, human tonsil tissue, SiHa. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:100-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q15021 Human |
| Alternative names: | 2810406C15Rik 2810465G24Rik CAP-D2 CAPD2 CEP2 CEP250 Chromosome condensation-related SMC-associated protein 1 Chromosome-associated protein D2 CNAP1 CND1 Condensin Condensin complex subunit 1 eg7 hCAP-D2 hCAPD2 KIAA0159 mKIAA0159 NCAPD2 non-SMC condensin I complex, subunit D2 XCAP D2 homolog |
Images
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Fig1:
Western blot analysis of NCAPD2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720100, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HL-60 cell lysate Lane 3: SiHa cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAPD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720100, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
ICC staining of NCAPD2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720100, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). Secondary antibody only control: Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.