Kallikrein 8 Recombinant Rabbit Monoclonal Antibody [JE62-46]
cat.: HA720101
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Kallikrein 8 aa 161-260/260. |
| Positive control: | A431 cell lysate, Jurkat cell lysate, Hela cell lysate, human kidney tissue. |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O60259 Human |
| Alternative names: | Brain serine protease 1 BSP1 hK8 HNP HPN Kallikrein 8 (neuropsin/ovasin) Kallikrein related peptidase 8 Kallikrein-8 Kallikrein8 KLK 8 Klk8 KLK8 protein type 1 KLK8 protein type 2 KLK8_HUMAN Neuropsin neuropsin type 1 Neuropsin type 1, included Neuropsin type 2 Neuropsin type 2, included Neuropsin, mouse, homolog of NP NRPN Ovasin PRO322 Protease serine 19 PRSS 19 PRSS19 Serine protease 19 Serine protease kallikrein Serine protease kallikrein/ovasin/neuropsin Serine protease TADG 14 Serine protease TADG-14 Serine protease TADG14 TADG 14 TADG14 Tumor associated differentially expressed gene 14 Tumor associated differentially expressed gene 14 protein Tumor-associated differentially expressed gene 14 protein UNQ283 |
Images
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Fig1:
Western blot analysis of Kallikrein 8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720101, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Predicted band size: 28 kDa Observed band size: 35 kDa (Glycosylation) |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Kallikrein 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720101, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.