PKN2 Recombinant Rabbit Monoclonal Antibody [JE62-44]
cat.: HA720102
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE62-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PKN2 aa 885-984/984. |
| Positive control: | A549 cell lysate, A431 cell lysate, Rat lung tissue lysate, human bladder tissue, mouse stomach tissue, rat stomach tissue, SiHa. |
| Subcellular location: | Cytoskeleton, Nucleus, Cytoplasm, Membrane, lamellipodium, Cleavage furrow, Midbody, Cell junction. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:100-1:500 1:50 |
| Uniprot #: | SwissProt: Q16513 Human | Q8BWW9 Mouse | O08874 Rat |
| Alternative names: | Cardiolipin activated protein kinase Pak2 MGC150606 MGC71074 PAK 2 PAK2 PKN gamma PKN2 PKN2_HUMAN PRK2 PRKCL2 PRO2042 Protein kinase C like 2 Protein kinase C related kinase 2 Protein kinase C-like 2 Protein kinase N2 Protein-kinase C-related kinase 2 Serine/threonine-protein kinase N2 |
Images
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Fig1:
Western blot analysis of PKN2 on different lysates with Rabbit anti-PKN2 antibody (HA720102) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PKN2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 112 kDa Observed band size: 112 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720102) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PKN2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720102, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Rat lung tissue lysate Predicted band size: 112 kDa Observed band size: 135 kDa |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-PKN2 antibody (HA720102) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720102) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PKN2 antibody (HA720102) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720102) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-PKN2 antibody (HA720102) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720102) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
ICC staining of PKN2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720102, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). Secondary antibody only control: Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.