PKN2 Recombinant Rabbit Monoclonal Antibody [JE62-44]
cat.: HA720102
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PKN2 aa 885-984/984. |
| Positive control: | A431 cell lysate, rat lung tissue lysate, rat bladder tissue, SiHa. |
| Subcellular location: | Cytoskeleton, Nucleus, Cytoplasm, Membrane, lamellipodium, Cleavage furrow, Midbody, Cell junction. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50 1:100-1:500 |
| Uniprot #: | SwissProt: Q16513 Human | O08874 Rat |
| Alternative names: | Cardiolipin activated protein kinase Pak2 MGC150606 MGC71074 PAK 2 PAK2 PKN gamma PKN2 PKN2_HUMAN PRK2 PRKCL2 PRO2042 Protein kinase C like 2 Protein kinase C related kinase 2 Protein kinase C-like 2 Protein kinase N2 Protein-kinase C-related kinase 2 Serine/threonine-protein kinase N2 |
Images
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Fig1:
Western blot analysis of PKN2 on different lysates with Rabbit anti-PKN2 antibody (HA720102) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PKN2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 112 kDa Observed band size: 112 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720102) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PKN2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720102, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Rat lung tissue lysate Predicted band size: 112 kDa Observed band size: 135 kDa |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-PKN2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720102, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
ICC staining of PKN2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720102, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). Secondary antibody only control: Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.