ENPP5 Recombinant Rabbit Monoclonal Antibody [JE62-55]
cat.: HA720103
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE62-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ENPP5 aa 378-477/477. |
| Positive control: | SK-Br-3 cell lysate, 293 cell lysate, MCF-7 cell lysate, mouse testis tissue lysate, SH-SY5Y, SiHa. |
| Subcellular location: | Secreted, Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UJA9 Human | Q9EQG7 Mouse |
| Alternative names: | E-NPP 5 E-NPP5 ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function) Ectonucleotide pyrophosphatase/phosphodiesterase 5 Ectonucleotide pyrophosphatase/phosphodiesterase family member 5 ENPP5 ENPP5_HUMAN NPP-5 UNQ550/PRO1107 |
Images
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Fig1:
Western blot analysis of ENPP5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720103, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: 293 cell lysate Lane 3: MCF-7 cell lysate Lane 4: Mouse testis tissue lysate |
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Fig2:
ICC staining of ENPP5 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720103, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). Secondary antibody only control: Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Flow cytometric analysis of ENPP5 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720103, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.