ALDH16A1 Recombinant Rabbit Monoclonal Antibody [JE62-68]
cat.: HA720104
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE62-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ALDH16A1 aa 550-600/802. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q8IZ83 Human | Q571I9 Mouse | Q3T1L0 Rat |
| Alternative names: | Aldehyde dehydrogenase 16 family, member A1 Aldehyde dehydrogenase family 16 member A1 ALDH16A1 aldehyde dehydrogenase 16 family, member A1 Aldh16a1 MGC10204 |
Images
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Fig1:
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody (HA720104) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 75 kDa Exposure time: 1 minute 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720104) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody (HA720104) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ALDH16A1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 80 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720104) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.