ALDH16A1 Recombinant Rabbit Monoclonal Antibody [JE62-68]
cat.: HA720104
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ALDH16A1 aa 550-600/802. |
| Positive control: | Daudi cell lysate, MCF-7 cell lysate, rat spleen tissue lysate, human small intestine tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q8IZ83 Human | Q571I9 Mouse | Q3T1L0 Rat |
| Alternative names: | Aldehyde dehydrogenase 16 family, member A1 Aldehyde dehydrogenase family 16 member A1 ALDH16A1 aldehyde dehydrogenase 16 family, member A1 Aldh16a1 MGC10204 |
Images
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Fig1:
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody (HA720104) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ALDH16A1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 80 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720104) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ALDH16A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720104, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: MCF-7 cell lysate Lane 3: Rat spleen tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ALDH16A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720104, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.