| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE62-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRPV5 aa 1-150/729. |
| Positive control: | SiHa cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, rat kidney tissue lysates. |
| Subcellular location: | Apical cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9NQA5 Human | P69744 Mouse | Q9JIP0 Rat |
| Alternative names: | Calcium transport protein 2 Calcium transporter 2 CAT 2 CAT2 ECAC 1 ECaC ECaC1 Epithelial calcium channel 1 Osm 9 like TRP channel 3 Osm-9-like TRP channel 3 OTRPC 3 OTRPC3 Transient receptor potential cation channel subfamily V member 5 TRPV 5 TrpV5 TRPV5_HUMAN |
|
Fig1:
Western blot analysis of TRPV5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: HL-60 cell lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse lung tissue lysate |
|
Fig2: Western blot analysis of TRPV5 on rat kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |