TRPV5 Recombinant Rabbit Monoclonal Antibody [JE62-37]
cat.: HA720109
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE62-37
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Recombinant protein within human TRPV5 aa 1-150/729.
Positive control: SiHa cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, rat kidney tissue lysates.
Subcellular location: Apical cell membrane.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q9NQA5 Human | P69744 Mouse | Q9JIP0 Rat
Alternative names: Calcium transport protein 2 Calcium transporter 2 CAT 2 CAT2 ECAC 1 ECaC ECaC1 Epithelial calcium channel 1 Osm 9 like TRP channel 3 Osm-9-like TRP channel 3 OTRPC 3 OTRPC3 Transient receptor potential cation channel subfamily V member 5 TRPV 5 TrpV5 TRPV5_HUMAN
Images
HA720109_1.jpg Fig1: Western blot analysis of TRPV5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: HL-60 cell lysate
Lane 3: Mouse kidney tissue lysate
Lane 4: Mouse lung tissue lysate
HA720109_2.jpg Fig2: Western blot analysis of TRPV5 on rat kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.