TRPV5 Recombinant Rabbit Monoclonal Antibody [JE62-37]
cat.: HA720109
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE62-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRPV5 aa 1-150/729. |
| Positive control: | SiHa cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, rat kidney tissue lysates. |
| Subcellular location: | Apical cell membrane. |
| Recommended Dilutions:
WB |
1:5,000-1:20,000 |
| Uniprot #: | SwissProt: Q9NQA5 Human | P69744 Mouse | Q9JIP0 Rat |
| Alternative names: | Calcium transport protein 2 Calcium transporter 2 CAT 2 CAT2 ECAC 1 ECaC ECaC1 Epithelial calcium channel 1 Osm 9 like TRP channel 3 Osm-9-like TRP channel 3 OTRPC 3 OTRPC3 Transient receptor potential cation channel subfamily V member 5 TRPV 5 TrpV5 TRPV5_HUMAN |
Images
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Fig1:
Western blot analysis of TRPV5 on different lysates with Rabbit anti-TRPV5 antibody (HA720109) at 1/20,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: SiHa (Human cervical cancersSquamous cell) cell lysate Lane 3: HL-60 (Human acute promyelocytic leukemia cell) cell lysate Lane 4: Mouse kidney tissue lysate(20 µg/Lane) Lane 5: Mouse heart tissue lysate(20 µg/Lane) Lane 6: Rat kidney tissue lysate(20 µg/Lane) Lane 7: rat heart tissue lysate(20 µg/Lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720109, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 82.6kDa Observed band size: 100kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.