HA720113

UQCRH Recombinant Rabbit Monoclonal Antibody [JE62-25]

cat.: HA720113

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE62-25

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 11 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human UQCRH aa 42-91/91.
Positive control:	Saos-2 cell lysate, Huh7 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse stomach tissue lysate, human kidney tissue, human stomach tissue, human small intestine tissue, mouse small intestine tissue, rat bladder tissue, THP-1.
Subcellular location:	Mitochondrion inner membrane.
Recommended Dilutions: WB IHC-P FC	1:1,000 1:400 1:500-1:1,000
Uniprot #:	SwissProt: P07919 Human \| P99028 Mouse \| Q5M9I5 Rat
Alternative names:	Complex III subunit 6 Complex III subunit VIII Cytochrome b c1 complex subunit 6 Cytochrome b c1 complex subunit 6 mitochondrial Cytochrome c1 non heme 11 kDa protein High protein , mitochondrial MGC111572 Mitochondrial hinge protein QCR 6 QCR6 Ubiquinol cytochrome c reductase complex 11 kDa protein Ubiquinol cytochrome c reductase complex 11 kDa protein mitochondrial Ubiquinol cytochrome c reductase complex III subunit VIII Ubiquinol cytochrome c reductase hinge protein ubiquinol-cytochrome c reductase hinge protein UQCR8

Images

	Fig1: Western blot analysis of UQCRH on different lysates with Rabbit anti-UQCRH antibody (HA720113) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 2: Mouse stomach tissue lysate (40 µg/Lane) Predicted band size: 11 kDa Observed band size: 14 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720113) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UQCRH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA720113, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-UQCRH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA720113, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-UQCRH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA720113, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-UQCRH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA720113, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-UQCRH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA720113, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of UQCRH was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720113, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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